| How to Ruin the Holidays (2023) Suzy |
| Pickled (2022) Self |
| Speech & Debate: Legacy (2021) Dr. Kandice Lewis |
| Untitled Horror Movie (2021) Bobbie Brower |
| Friendsgiving (2020) Lauren |
| Speech & Debate: The Aftermath (2020) Dr. Kandice Erisha Lewis |
| Speech & Debate (2020) Dr. Kandice Erisha Lewis |
| Bad Therapy (2020) Roxy |
| Stucco (2019) Realtor |
| Poop Talk (2018) Self |
| Axis (2017) Louise (voice) |
| Ear Buds: The Podcasting Documentary (2016) Herself |
| Adventures in Comedy (2015) |
| Comedy Central's All-Star Non-Denominational Christmas Special (2014) Herself |
| Why We Laugh: Funny Women (2013) Self |
| The Babymakers (2012) Karen |
| Another Dirty Movie (2012) Yayomi Finnigan (uncredited) |
| Black Water Transit (2009) Casey Spandau |
| Aisha Tyler Is Lit: Live at the Fillmore (2009) Herself |
| Bedtime Stories (2008) Donna Hynde |
| History of the Joke (2008) Self |
| The Trap (2007) Angela |
| Death Sentence (2007) Detective Wallis |
| Balls of Fury (2007) Mahogany |
| .45 (2006) Liz |
| The Santa Clause 3: The Escape Clause (2006) Mother Nature |
| For One Night (2006) Desiree Howard |
| Kanye West: College Dropout [Video Anthology] (2005) Self (segment "Slow Jamz") |
| Never Die Alone (2004) Nancy |
| Meet Market (2004) Jane |
| The Santa Clause 2 (2002) Mother Nature |